PMC

Pathways enriched among the key targets of the yin nourishing and heat clearing prescription in treating CVA.

Detection of single mRNAs with improved SNR. (A) The relative intensity of a single nanoprobe to cell background in a cellular region of interest (ROI) before optical clearing. (B) After clearing treatments, the SNR in cytoplasmic ROI was substantially enhanced. (C) Three key mRNAs relating to gynecological cancers were individually imaged in ER-expressing HeLa cells. Centroid images were reconstructed from the raw images by hyperspectral processing. (Scale bars: 10 μm)

Type I: (a) power-angle relation of P_E versus δ, (b) stability index for different clearing times showing a bipartite behaviour and (c) time-series diagrams. In (a), the red (blue) area A (B) denotes acceleration (deceleration) energy. Based on the EAC, we get the critical clearing angle and the corresponding CCT ≈ 0.136, as shown in (b), where if the fault clearing time is less than the CCT, the system could be stable, indicated by 0, or otherwise, indicated by 1. In (c), numerical results for two different clearing times (higher or lower than the CCT) prove this point well. The parameters here are: P_m = 0.65 and P_d = 0.6. The other two key parameters, P_b and P_p, are fixed throughout the paper: P_b = 1.3 and P_p = 0.8. The critical conditions obtained in the paper for various patterns of CCT should be carefully examined by moving the position of δ_c. In addition, we use solid grey lines in (a) for these critical conditions from analyses, where the definitions of α and β are in equations (3.4) and (3.8).

Analysis of whole mount NMJ morphology and quantification of NMJ numbers is enabled in wildtype and mdx muscles upon clearing. EDL muscles from wildtype (A,A',C,D) and dystrophic mdx mice (B,B',E,F) were processed using the MYOCLEAR protocol and stained with BGT-AF647. (A,B) Maximum-z projections of all NMJs detected by hand segmentation. Each cyan spot represents a single NMJ. (A',B') High-power images of some representative NMJs from each muscle shown in (A,B). (C,E) Upper panels, maximum-z projections of representative muscles showing BGT-staining signals. Lower panels, high power display of ROIs 1–5 in corresponding upper panel. (D,F) Quantitative analysis of key morphological parameters: area, fragmentation index, perimeter, and bounding rectangle diagonal of NMJs. Depicted are mean ± SD for all en face NMJs detected as a function of ROI number. ^*p < 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001, ^****p ≤ 0.0001.